Regulatory

Part:BBa_K4613429

Designed by: Qinfei Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)


PH207

Strong constitutive promoter, NAU-CHINA 2023 used this promoter in constructing BBa_K4613462, enabling PAL to have sufficient expression, exerting regulatory functions, and ensuring the normal operation of the system.

Reference

  1. Bubnov D M, Yuzbashev T V, Khozov A A, et al. Robust counterselection and advanced λRed recombineering enable markerless chromosomal integration of large heterologous constructs[J]. Nucleic Acids Research, 2022, 50(15): 8947–8960.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 61
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None